Atamt2 1
WebThe war against enormous humanoid beasts known as Titans continues in Season 2. After fighting the Female Titan, Eren must battle once again as a horde approaches Wall … WebFeb 7, 2000 · 3.1 Isolation of AtAMT2 and functional complementation of MLY131a/α, a yeast mutant deficient in ammonium uptake. The starting point for this work was an Arabidopsis genomic sequence (AC003028, sequence F16M14.22) that encoded a hypothetical protein with weak homology to plant ammonium transporters of the AMT1 …
Atamt2 1
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WebMar 30, 2024 · Giehl et al. (2024) reported that AtAMT2;1 makes a small but important contribution to ammonium uptake in the millimolar range of substrate. In plants subjected to high ammonium concentration, AMT2;1 mediates a high accumulation ammonium in xylem sap, although no net ammonium influx, which contributes to long-distance translocation … WebMar 1, 2024 · AtAMT2.1 is a high-affinity ammonium transporter with a Km value of about 20 μM (Sohlenkamp et_al, 2000; Sohlenkamp et_al, 2002), but most of the AMT2 members are low-affinity ammonium transporters. Whereas when the concentration is higher than 1 mM, LATS is activated and plays a role in absorbing NH4+, with a linear unsaturated …
WebMay 14, 2024 · Dynamic patterns of AtAMT1;3-dependent root ammonium uptake and T464 phosphorylation in response to ammonium and nitrate resupply. Six-week-old hydroponically grown Arabidopsis mutant qko+13 (atamt1;1, atamt1;2 and atamt2;1) were subjected to N starvation for 4 d, and then resupplied with 4 mM NH 4 Cl or 4 mM KNO 3 for 5, 15, and … WebSep 3, 2009 · Abstract. Prokaryotes, plants and animals control ammonium fluxes by the regulated expression of ammonium transporters (AMTs) and/or the related Rhesus (Rh) proteins. Plant AMTs were previously reported to mediate electrogenic transport. Functional analysis of AtAMT2 from Arabidopsis in yeast and oocytes suggests that NH (4) (+) is the ...
WebHome: Cell Press WebHowever, like AtAMT2 from Arabidopsis, and unlike AMT1 transporters from several plant species, LjAMT2;1 was unable to transport methylammonium. The LjAMT2;1 gene was found to be expressed constitutively throughout Lotus plants. In situ RNA hybridisation revealed LjAMT2;1 expression in all major tissues of nodules.
Webmembranes and probed with a 1.5 kb fragment of AtAMT2 cDNA, as described previously [24]. As a loading control, translation initia-tion factor elF4a [25] was used. 3. Results and discussion 3.1. Isolation of AtAMT2 and functional complementation of MLY131a/K, a yeast mutant de¢cient in ammonium uptake The starting point for this work was an ...
WebFeb 11, 2000 · Abstract. We have cloned and characterized the first member of a novel family of ammonium transporters in plants: AtAMT2 from Arabidopsis thaliana. AtAMT2 is more closely related to bacterial ammonium transporters than to plant transporters of the AMT1 family. The protein was expressed and functionally characterized in yeast. shanghai restaurant bristol vaWebMar 30, 2024 · AtAMT2, the sole member of the AMT2 subfamily in Arabidopsis, is strongly expressed in the root as well. While expression in the young root parts can be seen in the trichoblasts and root-hair (Neuhäuser et al. 2009 ), localization in the older root parts shifts to a preferential expression in the pericycle (Giehl et al. 2024 ). shanghai restaurant centralia waWebMay 14, 2024 · atamt1;2 and atamt2;1) were subjected to N starvation for 4 d, and then . resupplied with 4 mM NH 4 Cl or 4 mM KNO 3 for 5, 15, and 30 min. (A) shanghai restaurant cambridge ontarioWebFeb 11, 2000 · 3.1. Isolation of AtAMT2 and functional complementation of MLY131a/α, a yeast mutant deficient in ammonium uptake. The starting point for this work was an … shanghai restaurant brantfordshanghai restaurant cary ncWebMar 27, 2009 · A, Growth of NH 4 + uptake-deficient yeast (31019b; ΔΔΔmep1;2;3) transformed with NeRh-1, AtAMT1;2, AtAMT2;2, LjAMT2;2, and the empty control plasmid pDR199 on 3 m m ammonium as sole N source at different pH. Shown are serial dilutions (dil.) of cell suspensions ranging from 1 to 1 × 10 −4. shanghai restaurant clute texasWebNov 18, 2024 · The estimated K m value of ScAMT2;1 is higher than those determined for AtAMT1;1 and AtAMT2;1 via 13 N-ammonium in yeast (22 and 21 µM at pH 6.1, respectively) (Sohlenkamp et al., 2002). However, despite the similar K m values, the ammonium transport capacity of AtAMT2;1 was at least 10 times lower than that of … shanghai restaurant east london menu