WebCXXC finger protein 1 (Cfp1) is a regulator of both cytosine methylation and histone methylation. Murine embryonic stem (ES) cells lacking Cfp1 exhibit a decreased plating efficiency, decreased cytosine methylation, elevated global levels of histone H3-Lys4 trimethylation, and a failure to differentiate in vitro. WebCfp1. ORF names. CG17446. Organism names. Organism. Drosophila melanogaster (Fruit fly) Taxonomic identifier. 7227 NCBI. Taxonomic lineage. Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > …
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WebJul 28, 2024 · CFP1 is involved in multiple epigenetic signaling events linked to cancer by forming different protein complexes mediated by one, or several, of its regulatory … WebAug 1, 2024 · CXXC finger protein-1 (CFP1), encoded by the Cxxc1 gene in mice, is a key component of the eukaryotic SETD1 complex ( Clouaire et al., 2012 , Lee and Skalnik, … chancellor state college teachers
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WebMay 5, 2024 · In addition, CXXC finger protein 1 (CFP1) recruits the Set1 complex to CGIs ( 10 ), where this complex catalyzes methylation of histone H3 on lysine 4 (H3K4). Trimethylated H3K4 (H3K4me3) is generally associated with active transcription and potentially inhibits DNA methylation by preventing the recruitment of de novo DNA … CFP1-deleted cells were created using CRISPR/Cas9 [45]. Transient CRISPR plasmids pX459-Cfp1-1 and pX459-Cfp1-2 (pX459; plasmid #48139, Addgene, Watertown, MA, USA) were prepared for CFP1 knock-out to avoid unspecific effects mediated by Cas9/single-guide RNA (sgRNA). Human Cfp1 sgRNA sequences … See more Human ovarian cancer cell lines A2780, ES-2, OVCAR-3, HO8910, CAOV3, and SKOV3 were purchased from ATCC (Manassas, VA, USA) and IOSE cells were supplied by … See more Six-well plates were coated with 1.5 mL of 0.5% bottom agar (Sigma-Aldrich, St. Louis, MO, USA) in a 35-mm diameter culture dish (Corning, Corning, NY, USA). Cells were suspended in 1 mL of 0.35% agar containing 1× … See more A BST2 overexpression vector was constructed using a pQCXIH retroviral vector (Addgene, plasmid #17394) containing the full-length cDNA sequence of BST2. A total of … See more ES-2 WT and CFP1-deleted cells were grown to confluence in DMEM supplemented with 10% FBS in six-well plates. Then, the medium was changed to FBS-free DMEM, and the cell monolayers were scraped in a … See more WebCarried out multiple fundraising events, including these: 2014 - 5 Day cycling event, organising and leading a team of 7 on 4 days cycling from Portsmouth to Snowden, and then climbing Mount Snowden on the final day. 2015 - 5 Days cycling event, organising and leading a team of 8 from Portsmouth to Paris and back again, including climbing the ... harbor cleveland