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Cisplatin binding to holliday junction

WebK Komori, S Sakae, H Shinagawa, K Morikawa, Y Ishino, A Holliday junction resolvase from Pyrococcus furiosus: Functional similarity to Escherichia coli RuvC provides … WebOct 5, 2014 · 1. Introduction. Cisplatin (CAS No. 15663-27-1, MF-Cl 2 H 6 N 2 Pt; NCF-119875), cisplatinum, also called cis-diamminedichloroplatinum(II), is a metallic (platinum) coordination compound with a square planar geometry.It is a white or deep yellow to yellow-orange crystalline powder at room temperature. It is slightly soluble in water and soluble …

Ligand Bridging of the DNA Holliday Junction: Molecular …

WebJun 3, 2024 · Wang, W. et al. Holliday junction thermodynamics and structure: coarse-grained simulations and experiments. Sci. Rep. 6, 22863 (2016). WebAug 5, 2011 · Holliday junctions are formed during recombination, double-strand break repair, and fork reversal during replication. Resolving Holliday junctions is a critical process for maintaining genomic stability [ 25, 26 ]. These junctions are resolved by a class of structure-specific nucleases: the junction-resolving enzymes. Figure 1 land match https://jirehcharters.com

Structure and subunit composition of the RuvAB-Holliday junction ...

WebThe BCDX2 complex also bound to the synthetic Holliday junction, and its af®nity to the Holliday-junction DNA was signi®cantly higher than that of Rad51B (Fig. 4). WebAbstract. In the present paper, the interactions of the origin binding protein (OBP) of herpes simplex virus type 1 (HSV1) with synthetic four-way Holliday junctions (HJs) were … WebBinding of RuvA to the Holliday Junction109 phosphate atoms across the minor groove in B-DNA. It is tempting to speculate that the arrangement of the two approximately 2-fold- related HhH... land mate beecroft

Holliday junction binding by the BCDX2 complex. (A) The …

Category:Binding to four-way junction DNA: a common property of

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Cisplatin binding to holliday junction

Resolution of single and double Holliday junction recombination

WebConformational switching of the synthetic Holliday junction (HJ) labeled with a Cy3-Cy5 fluorophore pair. (b) Representative single molecule fluorescence-time traces from our … WebSep 15, 2024 · Cisplatin (Cisplatin Injection) may treat, side effects, dosage, drug interactions, warnings, patient labeling, reviews, and related medications including drug …

Cisplatin binding to holliday junction

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WebMar 15, 1997 · The results confirm that RuvC protein binds Holliday junctions as a dimer. Influence of DNA sequence on incision Previously it was shown that Holliday junction cleavage by RuvC occurs at specific sequences, with the consensus defined as 5′- A / T TT ↓G / C -3′. Single base changes within this sequence severely reduce the efficiency of … WebThe RuvAB complex can carry out DNA helicase activity, which helps unwind the duplex DNA. The binding of the RuvC protein to the RuvAB complex is thought to cleave the …

WebFeb 16, 2024 · severe or ongoing nausea and vomiting; vision problems, pain behind your eyes; hearing problems, ringing in your ears; pain, redness, swelling, or skin changes … WebFour-way DNA intermediates, called Holliday junctions (HJs), can form during meiotic and mitoticrecombination,andtheirremovaliscrucialforchromosomesegregation.Agroupof ubiquitous and highly specialized structure-selective endonucleases catalyze the cleavage of HJs into two disconnected DNA duplexes in a reaction called HJ resolution.

WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … Webformation of Holliday junction 1. after strand invasion, the two DNA molecules are connected by crossing DNA strands to form the Holliday junction 2. junction can move along the DNA by repeated DNA melting and formation of base pairs 3. process is called branch migration resolution of Holliday junction

WebJul 2, 2004 · The RuvC protein binds specifically to a Holliday junction and cleaves it in one of the two possible orientations [16], [17], [18]. No spontaneous or cisplatin-induced …

WebTo expose two cut sites, the Holliday junction is rotated by 180° to form a sequare planar structure. Resolution of Holliday junction occurs by cutting the DNA strands at the site of cross and re-joining them. Resolution occurs in one of the two ways. This gives rise to two classes of DNA products. landmaster weed control fabricWebJul 11, 2000 · Actually, both of the RuvA tetramers, the upper and the lower, simultaneously contact each strand of a DNA duplex arm ( 16 ). By contrast, in complex I, the two … he-man masters of the universe season 3WebDNA polymerases fill in 3′ overhangs, whereby the D-loop becomes a Holliday junction. RAD52 functions to anneal the second end of DNA leading to a Holliday junction. BLM migrates the two ends of the Holliday junction in the direction of one another with some filling in by polymerases if necessary. landmasters syracuse