Opti mem thermo
WebAug 4, 2024 · For solution 2, 5 µL Lipofectamine® 2000 reagent (Invitrogen, Waltham, MA, USA) was mixed with 250 µL Opti-MEM and incubated for 5 minutes. ... (100 µL) was added to each well and incubated in a cell incubator for 4 hours. A microplate reader (Thermo Fisher Scientific) was used to detect the absorbance at 450 nm. Cell viability was ... WebPublication Number MAN0007448 Rev. 1.01 Opti-MEM® I Reduced Serum Medium Description Opti-MEM® I Reduced-Serum Medium is a chemically defined, low protein, Minimal Essential Medium (MEM) containing insulin, transferrin, hypoxanthine, thymidine, and trace elements th at allows for a reduction of Fetal Bovine Serum supplementation by …
Opti mem thermo
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WebJan 28, 2024 · Dilute 30 μg of DNA plasmid into 1.5 mL of Opti-MEM Reduced Serum Medium and mix gently. (b) Concomitantly, dilute 80 μL of ExpiFectamine 293 reagent in Opti-MEM Reduced Serum to a total volume of 1.5 mL, mix gently, and incubate at room temperature for 5 min. (c) WebOpti-MEM reduced serum medium, no phenol red (Life Technologies, Cat. No. 11058-021) 3. Sucrose (Sigma-Aldrich, Cat. No. S0389) 4. 0.1% fibronectin solution from human plasma (Sigma-Aldrich, Cat. No. F0895) 5. Gelatin powder from bovine skin (Sigma-Aldrich, Cat. No. G9391) 6. RNase-free water (Fisher BioReagents, Cat. No. 7732-18-5) 7.
WebOpti-MEM™ I Reduced Serum Medium (Cat. no. 31985) ∤ Eppendorf tubes Timing ∤ Preparation: 10 minutes ∤ Incubation: 5 + 10 minutes ∤ Final Incubation: 2–3 days … WebThermo Fisher opti mem serum free media Opti Mem Serum Free Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.
WebMar 16, 2024 · Base editors (BEs) are CRISPR-based genome editing tools where nuclease-impaired Cas9 directs a deaminase to the locus of interest to install single-nucleotide conversions. They enable precise genome editing independent of HDR ( 9, 10) and also operate with high efficiency and accuracy in somatic tissues in vivo ( 11 – 17 ). WebOpti-MEM ™ I Reduced Serum Medium. Opti-MEM ™ I Reduced Serum Medium is a serum-free medium used to complex plasmid DNA with ExpiFectamine ™ 293 Reagent, providing …
WebSep 30, 2024 · All siRNAs used in this study were obtained from RIBO Biotechnology (China) and transfected into CCA cells using Lipotransfectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) according to the instructions. Briefly, CCA cells were seeded in a six-well plate until the cell density reached approximately 60%. 200 μL serum-free OPTI-MEM ...
WebMar 10, 2024 · BY-2-xGxFL cells were collected in a tube and washed once with Opti-MEM I (Thermo Fisher Scientific, Waltham, MA, USA). Cells with 20 µL of PCV were suspended in 200 µL of Opti-MEM I with HNCre protein on ice. HNCre protein concentrations were 5 µM to evaluate the poring pulse voltage and length effect, were 0.1, 0.2, 0.5, 1, 2, or 5 µM to ... chita and hayesWebOpti-MEM I medium 25 µL Lipofectamine 3000 reagent 2 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0.5–5 µg/µL) 250 ng P3000™ reagent 0.5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; graph todayWebWe offer a variety of Opti-MEM™ I Reduced Serum Medium modifications for a range of cell culture applications. Opti-MEM™ I Reduced Serum Medium is also recommended for use … chita awardsWebOpti-MEM® Reduced Serum Medium ∤ Eppendorf tubes Timing Preparation: 10 minutes Incubation: 5 minutes Final Incubation: 1-3 days Selection Guide Lipofectamine® Reagents Go online to view related products. Product Description ∤ Lipofectamine® LTX and PLUS™ Reagent are proprietary formulations for transfecting nucleic acids into a wide range chitabe botswanaWebFeb 12, 2024 · Opti-MEM (Thermo Fisher Scientific, Waltham, MA, USA). 8. Stereomicroscope. 9. Glass capillary pipettes. 10. Fluorescent inverted microscope. 11. ... in Opti-MEM medium. 3. Add 100 μg/mL of ssODN in the nuclease solution when produce knock-in strain. 4. Introduce 10 μL of nuclease solution into the electrode (Fig. 1b). 5. chita bat backpacksWebopti mem ( Thermo Fisher ) Thermo Fisher is a verified supplier Thermo Fisher manufactures this product About News Press Release Team Advisors Partners Contact Bioz Stars Bioz vStars 86 Buy from Supplier Opti Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. chita bar stoolsWebnepa21 高效基因转染系统(日本nepa gene)；olympus ix51倒置荧光显微镜(奥林巴斯)； nanodrop 2000超微量分光光度计(gene company limited)；galliostm流式细胞分析仪(beckman coulter)；varioskan flash全波长光谱扫描仪(thermo scientific)；js-680d全自动数码凝胶成像分析系统(上海培清科技有限 ... graph to data converter software