Phosphotyrosine 4g10
WebApr 17, 2007 · Bound proteins were eluted with 500 mmol/L Imidazole and fractionated through 12% SDS-PAGE before their electrophoretic transfer to nitrocellulose membranes. Immobilized proteins were probed sequentially with antibodies against phosphotyrosine (4G10 antibodies; Upstate, Charlottesville, VA) followed by those against GST (Invitrogen, … WebCells were lysed and underwent immunoprecipitation (IP) with antibody specific for Jak2 or Stat5; Western blot (WB) was then performed with antibodies against phosphotyrosine (4G10), total Jak2 ...
Phosphotyrosine 4g10
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WebChemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin. Positive control ICC/IF: C2C12 cells treated with 2mM H2O2 for 10min. WB: NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate. General notes This antibody clone is manufactured by Abcam.
WebJan 12, 2012 · Embryos were incubated with biotin-conjugated phosphotyrosine (anti-4G10, Millipore) antibody overnight at 4°C at a dilution of 1 : 500 (in block solution). Embryos were then washed (3 × 10 min) in PBDT and incubated for 2 hours at room temperature with streptavidin conjugated Cy3 or QDs 655 nm at 1 : 500 dilution in fresh block solution. WebPhospho-Tyrosine (4G10) Mouse mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides containing phospho-Ser or phospho-Thr residues. Species Reactivity: All Species Expected Source / Purification
WebApr 1, 2010 · Tyrosine phosphate is a frequently observed modification in a limited number of protein molecules, principally the products of viral oncogenes and membrane receptors for mitogenic growth factors.... WebPublished Ahead of Print 5 February 2007. €€ 10.1128/MCB.00079-07. Mol. Cell. Biol.€2007, 27(8):2830. DOI: Stephanopoulos, Lewis C. Cantley and C. Ronald Kahn
WebSep 19, 2016 · These data suggest that the Src superbinder may provide an alternative means to commonly used anti-phosphotyrosine antibodies 13 such as 4G10 and P-Tyr-100 for the purification of...
WebAnti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is … port isabel tx weather forecastWebAnti-Phosphotyrosine Antibody, clone 4G10® Anti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody … iro section 39eWebUpstate Biotechnology Inc anti phosphotyrosine ptyr antibody 4g10 Anti Phosphotyrosine Ptyr Antibody 4g10, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more port isabel veterinary clinicWeb16-316 Sigma-Aldrich Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate Download Zoom Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), … iro section 26aWebDec 14, 2024 · b Phosphotyrosine cell content was analysed by immunoblot with anti-phosphotyrosine 4G10 mAb (pY). GAPDH immunoblot was run as loading control. In the upper panel, a representative experiment is shown. In the lower panel, the quantification of phosphotyrosine content from each condition is shown. Results are shown as … iro selling wpeWebAntibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. port isabel tx lodgingWebAnti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA. Research Category Signaling Research Sub Category … port isabel tx winter rentals