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WebMar 23, 2024 · Peptides containing this di-glycine remnant after trypsin digest could then be purified using the K-ε-GG immunoaffinity enrichment pipeline established for ubiquitinated proteins, and processed to identify sites of diglycine modification on target proteins. WebTo identify putative effector proteins and establish sites of SUMO modification, cells were engineered to stably express either SUMO1 or SUMO2 with C-terminal TGG to KGG mutations that facilitate GlyGly-K peptide immunoprecipitation and identification. A total of 976 SUMO sites were identified in 427 proteins. how are ants important to the world
Site-specific identification and quantitation of endogenous …
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