Tpx2 phase
SpletPhase separation of substrates and effectors is proposed to enhance biological reaction rates and efficiency. TPX2 is an effector of microtubule nucleation in spindles, and … Splet21. dec. 2015 · TPX2 is a microtubule-associated protein that is required for mitotic spindle assembly and function. TPX2 has a nuclear localization sequence (NLS) and is nuclear …
Tpx2 phase
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Splet25. jun. 2024 · The results indicated that TPX2 arrested the cell cycle in G0 phase in the LNCaP cells, but not in the PC3 cells . Figure 3. TPX2 expression and function in prostate … SpletHere we show that TPX2 phase separates into a co-condensate with tubulin, which mediates microtubule nucleation in vitro and in isolated cytosol. TPX2-tubulin co …
Splettively active in Xenopus M-phase egg extract.3 Furthermore, TPX2 is a microtubule associated protein (MAP) and may participate in microtubule dynamics, bundling and/or … Splet03. okt. 2008 · TPX2 activity is tightly regulated at the protein level during the two successive meiotic divisions. In prophase I, the complete absence of TPX2 depends on …
Splet28. jan. 2024 · TPX2 was flash frozen in liquid nitrogen and stored at −80 °C until use. This TPX2 was used for all fluorescence, electron and atomic force microscopy experiments. SpletTPX2 levels steadily increase during cell cycle progression with highest expression levels in M phase and lowest levels in G1 phase. TPX2 activates mitotic serine-threonine Aurora A kinase, nucleates microtubules and is considered as a critical factor for spindle assembly in mammalian mitotic cells.
SpletTargeting protein for Xklp2 (TPX2) is an effector of branching microtubule nucleation in spindles and functions with the substrate tubulin by an unknown mechanism. Here we …
Splet01. sep. 2024 · TPX2 is a key factor for branching microtubule nucleation, as it initiates this reaction by binding to microtubules and marking the branch site to recruit the other … bdo audit graduateSpletInteraction of spindle assembly factor TPX2 with importins-α/β inhibits protein phase separation. The microtubule-based mitotic spindle is responsible for equally partitioning … bdo audit graduate salary ukSplet17. jul. 2024 · A Western blot analysis revealed that TPX2 protein expression was high at the G2/M phase, decreased at the G1 to S phase, increased at the beginning of S phase, and then peaked again at the next G2/M phase (Figure 3A). TPX2 depletion reduced the G1- and S-phase proportions; by contrast, inhibition of TPX2 expression resulted in high amounts … denis bećirović mirela bećirovićSplet17. feb. 2024 · 研究者King指出，研究结果表明，TPX2和微管蛋白的共缩作用能在已经存在的微管结构上形成一个局部的围观蛋白存储库，这或有望有效促进微管蛋白分支成核的 … denis bećirović jasminka bećirovićSplet03. nov. 2014 · Because TPX2's DNA damage response function is particularly evident during G1-phase (see ), we next determined the TPX2-dependent levels of H4K16ac at this cell cycle stage. To do so, we utilized the HeLa cell line expressing a doxycycline-inducible TPX2 miRNA and synchronized these cells with a double thymidine block [15] , [48] . denis beroš prehrana po krvnim grupamaSplet01. okt. 2024 · We highlight the roles of microtubule binding proteins tau, TPX2, and BuGZ. Tau is a microtubule-stabilizing protein that has been highly studied because aggregates of tau have been implicated in a number of neurodegenerative diseases ( Wang and Mandelkow, 2016 ). bdo audit firm pakistanSpletIn addition, we establish that TPX2 interacts with importin-β via dispersed, weak interactions. We show that interactions of both importin-α and -β with TPX2 inhibit its … bdo audit graduate salary london